2006, 2(2):158-162.
Abstract:
Charge recombination in reaction center (RC) of photosystem II(PS II) is regarded as the location of 685 nm delayed fluorescence
(DF). The mechanism of 730 nm component appearing in the DF spectrum for chloroplast was studied by various spectral analysis
methods. Experimental results of the DF spectrum at different chloroplast concentration show that the intensity of peaks at
685nm and 730 nm ascends with the chloroplast concentration increasing when the concentration is relatively low. When the
concentration increases to the level of 7.8μg/ml, a maximum intensity of the peak at 685 nm appears but the intensity of 730
nm peak still increases. The peak at 730 nm finally reaches a maximum intensity at the chloroplast concentration of 31.2 μg/ml
while the intensity of the 685 nm peak has apparently fallen down. The results of absorption spectrum show that the ratios
of A685 to A730 keep almost constant with the increasing of chloroplast concentration. Furthermore, the excitation spectrum
for 730 nm fluorescence shows that the 685nm light has high excitation efficiency. These results indicate that the 730 nm
component of DF spectrum is the fluorescence of chlorophyll in PS I RC excited by 685 nm DF. Meanwhile, this can be further
verified by the invariability of DF spectrum at different delay time (1 second∼9 seconds).
This research is supported by the National Natural Science Foundation of China (60378043), and supported by the Opening Foundation
of the Key Laboratory of Opto-Electronic Technology and Intelligent Control (Lanzhou Jiaotong University), Ministry of Education
(K04108)