A label-free lateral offset spliced coreless fiber MZI biosensor based on hydrophobin HGFI for TNF-α detection
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1. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300071, China;2. Institute of Modern Optics, Tianjin Key Laboratory of Optoelectronic Sensor and Sensing Network Technology, Nankai University, Tianjin 300350, China;3. School of Life Science, Shanxi University, Taiyuan 030006, China

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    Abstract:

    A real-time label-free lateral offset spliced coreless fiber (CF) Mach-Zehnder interferometer (MZI) biosensor functionalized with hydrophobin Grifola frondosa I (HGFI) was proposed for the detection of cytokine tumour necrosis factor alpha (TNF-α). The nanolayer self-assembled on the optical fiber surfaces by HGFI rendered the immobilization of probe TNF-α antibody and recognition of antigen TNF-α. Trifluoroacetic acid was utilized to remove the HGFI layer from the glass surface, which was validated by field emission scanning electron microscopy (FESEM) and water contact angle (WCA). Results demonstrated that the processes of HGFI modification, antibody immobilization and specific antibody detection can be monitored in real time. The proposed biosensor exhibited good specificity, repeatability and low detection limit for TNF-α, extending its application in inflammation and disease monitoring.

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WANG Bo, DUAN Shaoxiang, ZHANG Hao, XU Haijin, LIU Bo, QIAO Mingqiang. A label-free lateral offset spliced coreless fiber MZI biosensor based on hydrophobin HGFI for TNF-α detection[J]. Optoelectronics Letters,2022,18(5):263-268

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History
  • Received:April 13,2022
  • Revised:April 24,2022
  • Online: June 07,2022
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