Label-free quantitative detection using porous silicon as optical biosensor
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Abstract:
The single-layer porous silicon is prepared by electrochemistry etching method, which is used as an immunosensor for determining recombinant mouse zona pellucida 3 fusion protein (r-mZP3) by Raman spectroscopy analysis at room temperature. The molecule binding increases the effective optical thickness (EOT), and thus the Raman spectrum intensity decreases. The concentration and variation of Raman intensity show a good linear quantitative relation. The excellent sensing performance could open the way to a new family of optical sensors for biological standardization.
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This work has been supported by the National Natural Science Foundation of China (No.60968002), and the China Postdoctoral Science Foundation (No.2011M501501).
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