Time-resolved two-photon excitation fluorescence spectroscopy and microscopy using a high repetition rate streak camera

Abstract:

We present a time-resolved two-photon excitation fluorescence spectroscopy and a simultaneous time- and spectrumresolved multifocal multiphoton microscopy system that is based on a high repetition rate picosecond streak camera for providing time- and spectrum- resolved measurement and imaging in biomedicine. The performance of the system is tested and characterized by the fluorescence spectrum and lifetime analysis of several standard fluorescent dyes and their mixtures.Spectrum-resolved fluorescence lifetime images of fluorescence beads are obtained. Potential applications of the system include clinical diagnostics and cell biology etc.